Dextran-Coated Charcoal Technique to Make the Hormone-Free Serum as a Diluent for Standard Curve of Radioimmunoassay
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چکیده
منابع مشابه
Laboratory application of the dextran-coated-charcoal radioimmunoassay of insulin.
A modification of the method for radioimmunoassayof insulin is described, which achievessatisfactory sensitivity while employing commercially available reagents and antiserums. These modifications consist mainly in lowering the initial percentage binding, reducing the amount of labeled insulin, and preincubating the unlabeled hormone with antiserum. Application of the linear isotope dilution fo...
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Biopsy specimens from 21 women with primary operable breast cancer were analyzed in a double blind study designed to compare fluorescent antibody methods for detection of estrogen receptors with estrogen receptors measured biochemically with a previously reported dextran coated charcoal technique (DCC). Fluorescent antibody methoids employed both direct techniques: (1) rabbit antisera to 6-O-Ca...
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I describe a convenient method for stripping hormones and other ligands from human serum. Formation of a serum/charcoal colloid is avoided by incubating serum on a limited surface area with activated charcoal, in a lateral oscillating shaker. Particle-free serum of negligible turbidity is obtained without ultracentrifugation by ejecting the centrifugate through a cellulose ester membrane. By th...
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Human serum can be depleted of insulin and growth hormone by treatment with dextran-coated charcoal or by dialysis, or both. Inclusion of such hormone-depleted serum in the standard curve of a double-antibody radioimmunoassay for immunoreactive insulin more nearly mimics the conditions under which an unknown human serum sample is assayed. Compared to the usual standard curve in which serum albu...
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We describe the preparation and evaluation of a new diluent for use in preparing standard curves for the radioimmunoassay of human thyroglobulin (TG). This diluent, "TG-free human plasma, allows measurement of TG in lower concentrations than with previous assay systems because the components of the standards are more similar to those of the samples. TG-free plasma was prepared by a modification...
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ژورنال
عنوان ژورنال: Endocrinologia Japonica
سال: 1973
ISSN: 0013-7219,2185-6370
DOI: 10.1507/endocrj1954.20.1